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pentr ubiquitin c promoter  (Addgene inc)


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    Structured Review

    Addgene inc pentr ubiquitin c promoter
    Pentr Ubiquitin C Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+ubiquitin+c+promoter/pENTR-L4-UBI-L1R+(Plasmid+%2345959)/pmc08565315-56-18-22
    Average 92 stars, based on 3 article reviews
    pentr ubiquitin c promoter - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Recombinant:

    Article Title: ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72 -associated ALS/FTD
    Article Snippet: Recombinant lentiviral vectors for the pmGFP-P2A-K0-P2A-RFP and pmGFP-P2A-K(AAA)20-P2A-RFP reporters were constructed by the Gateway cloning of modified pENTR4 entry vectors (Addgene plasmid #17424) harboring the reporter cDNAs into pLenti-PGK-Puro-DEST destination vector (Addgene plasmid #19068). .. Recombinant lentiviral vectors for the overexpression of wild-type or mutant ZNF598 were constructed by the Gateway cloning of pENTR- Ubiquitin C promoter (Addgene plasmid #45959) and modified pENTR4-FLAG entry vectors (Addgene plasmid #17424) harboring individual ZNF598 cDNAs into pCWX-DEST destination vector (Addgene plasmid #45957). .. Human neuroblastoma SH-SY5Y cells and embryonic kidney 293T cells were cultured in Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum and 1% antibiotics.

    Over Expression:

    Article Title: ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72 -associated ALS/FTD
    Article Snippet: Recombinant lentiviral vectors for the pmGFP-P2A-K0-P2A-RFP and pmGFP-P2A-K(AAA)20-P2A-RFP reporters were constructed by the Gateway cloning of modified pENTR4 entry vectors (Addgene plasmid #17424) harboring the reporter cDNAs into pLenti-PGK-Puro-DEST destination vector (Addgene plasmid #19068). .. Recombinant lentiviral vectors for the overexpression of wild-type or mutant ZNF598 were constructed by the Gateway cloning of pENTR- Ubiquitin C promoter (Addgene plasmid #45959) and modified pENTR4-FLAG entry vectors (Addgene plasmid #17424) harboring individual ZNF598 cDNAs into pCWX-DEST destination vector (Addgene plasmid #45957). .. Human neuroblastoma SH-SY5Y cells and embryonic kidney 293T cells were cultured in Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum and 1% antibiotics.

    Mutagenesis:

    Article Title: ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72 -associated ALS/FTD
    Article Snippet: Recombinant lentiviral vectors for the pmGFP-P2A-K0-P2A-RFP and pmGFP-P2A-K(AAA)20-P2A-RFP reporters were constructed by the Gateway cloning of modified pENTR4 entry vectors (Addgene plasmid #17424) harboring the reporter cDNAs into pLenti-PGK-Puro-DEST destination vector (Addgene plasmid #19068). .. Recombinant lentiviral vectors for the overexpression of wild-type or mutant ZNF598 were constructed by the Gateway cloning of pENTR- Ubiquitin C promoter (Addgene plasmid #45959) and modified pENTR4-FLAG entry vectors (Addgene plasmid #17424) harboring individual ZNF598 cDNAs into pCWX-DEST destination vector (Addgene plasmid #45957). .. Human neuroblastoma SH-SY5Y cells and embryonic kidney 293T cells were cultured in Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum and 1% antibiotics.

    Construct:

    Article Title: ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72 -associated ALS/FTD
    Article Snippet: Recombinant lentiviral vectors for the pmGFP-P2A-K0-P2A-RFP and pmGFP-P2A-K(AAA)20-P2A-RFP reporters were constructed by the Gateway cloning of modified pENTR4 entry vectors (Addgene plasmid #17424) harboring the reporter cDNAs into pLenti-PGK-Puro-DEST destination vector (Addgene plasmid #19068). .. Recombinant lentiviral vectors for the overexpression of wild-type or mutant ZNF598 were constructed by the Gateway cloning of pENTR- Ubiquitin C promoter (Addgene plasmid #45959) and modified pENTR4-FLAG entry vectors (Addgene plasmid #17424) harboring individual ZNF598 cDNAs into pCWX-DEST destination vector (Addgene plasmid #45957). .. Human neuroblastoma SH-SY5Y cells and embryonic kidney 293T cells were cultured in Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum and 1% antibiotics.

    Cloning:

    Article Title: ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72 -associated ALS/FTD
    Article Snippet: Recombinant lentiviral vectors for the pmGFP-P2A-K0-P2A-RFP and pmGFP-P2A-K(AAA)20-P2A-RFP reporters were constructed by the Gateway cloning of modified pENTR4 entry vectors (Addgene plasmid #17424) harboring the reporter cDNAs into pLenti-PGK-Puro-DEST destination vector (Addgene plasmid #19068). .. Recombinant lentiviral vectors for the overexpression of wild-type or mutant ZNF598 were constructed by the Gateway cloning of pENTR- Ubiquitin C promoter (Addgene plasmid #45959) and modified pENTR4-FLAG entry vectors (Addgene plasmid #17424) harboring individual ZNF598 cDNAs into pCWX-DEST destination vector (Addgene plasmid #45957). .. Human neuroblastoma SH-SY5Y cells and embryonic kidney 293T cells were cultured in Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum and 1% antibiotics.

    Ubiquitin Proteomics:

    Article Title: ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72 -associated ALS/FTD
    Article Snippet: Recombinant lentiviral vectors for the pmGFP-P2A-K0-P2A-RFP and pmGFP-P2A-K(AAA)20-P2A-RFP reporters were constructed by the Gateway cloning of modified pENTR4 entry vectors (Addgene plasmid #17424) harboring the reporter cDNAs into pLenti-PGK-Puro-DEST destination vector (Addgene plasmid #19068). .. Recombinant lentiviral vectors for the overexpression of wild-type or mutant ZNF598 were constructed by the Gateway cloning of pENTR- Ubiquitin C promoter (Addgene plasmid #45959) and modified pENTR4-FLAG entry vectors (Addgene plasmid #17424) harboring individual ZNF598 cDNAs into pCWX-DEST destination vector (Addgene plasmid #45957). .. Human neuroblastoma SH-SY5Y cells and embryonic kidney 293T cells were cultured in Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum and 1% antibiotics.

    Plasmid Preparation:

    Article Title: ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72 -associated ALS/FTD
    Article Snippet: Recombinant lentiviral vectors for the pmGFP-P2A-K0-P2A-RFP and pmGFP-P2A-K(AAA)20-P2A-RFP reporters were constructed by the Gateway cloning of modified pENTR4 entry vectors (Addgene plasmid #17424) harboring the reporter cDNAs into pLenti-PGK-Puro-DEST destination vector (Addgene plasmid #19068). .. Recombinant lentiviral vectors for the overexpression of wild-type or mutant ZNF598 were constructed by the Gateway cloning of pENTR- Ubiquitin C promoter (Addgene plasmid #45959) and modified pENTR4-FLAG entry vectors (Addgene plasmid #17424) harboring individual ZNF598 cDNAs into pCWX-DEST destination vector (Addgene plasmid #45957). .. Human neuroblastoma SH-SY5Y cells and embryonic kidney 293T cells were cultured in Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum and 1% antibiotics.

    Modification:

    Article Title: ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72 -associated ALS/FTD
    Article Snippet: Recombinant lentiviral vectors for the pmGFP-P2A-K0-P2A-RFP and pmGFP-P2A-K(AAA)20-P2A-RFP reporters were constructed by the Gateway cloning of modified pENTR4 entry vectors (Addgene plasmid #17424) harboring the reporter cDNAs into pLenti-PGK-Puro-DEST destination vector (Addgene plasmid #19068). .. Recombinant lentiviral vectors for the overexpression of wild-type or mutant ZNF598 were constructed by the Gateway cloning of pENTR- Ubiquitin C promoter (Addgene plasmid #45959) and modified pENTR4-FLAG entry vectors (Addgene plasmid #17424) harboring individual ZNF598 cDNAs into pCWX-DEST destination vector (Addgene plasmid #45957). .. Human neuroblastoma SH-SY5Y cells and embryonic kidney 293T cells were cultured in Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum and 1% antibiotics.



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